Foothill trail

I found a new biking trail today about 8 miles from home. Foothill is by the highway 280, and it is not difficult to climb up to the top since the height goes up smoothly, but still I have to walk my single gear bike sometimes. Yet, it is ecstatic to bike down the hill on the road, so it is worth the strength anyway. Unexpected, I met four deers on the hill, they were hiding behind the tree from sunshine or predators? They were not afraid of me at all.

Fremont older open space reserve & Stevens Creek County Park trail

I found another two biking trails about 8 miles from home. One is Fremont older open space reserve on hills, the other is Stevens Creek Country Park in the valley.  They are neighbors, so it's easy to ride one time for both places. After several days in flat Silicon Valley, I had some fun on hills for the first time, even though I had to walk my steel bike on some steep roads.

Steven Creek Trail to Bay trail

I found a lovely five-mile bike trail to the bay shore, along the Stevenson creek, with two lanes, each for one direction. Trees are all the way down to the bay, and many beautiful crossing bridges make it even scenic. The speed limit is 5mph so I cannot ride fast. When I reached the end, the trail is on a narrow dam, still two lanes. I felt like flying off because of its height and the surrounding emptiness.

Family tree of neurons in the brain

Darwin built the tree of evolution, where we learn our position in the history of life on Earth. You can build your family tree with your clan pedigree books. A recent paper in Science reports building a linage tree for neurons.

Archeologists use radioactive isotopes to estimate the age of a rock or bone; biologists use DNA labels to trace cell division;  with genome sequencing, geneticists can use gene mutations to trace our ancestors to African apes. In the Science paper, Lodato et al. used whole genome sequencing of single neurons to detect the mutations with which they built a linage tree of neurons for a single person.

We know during early development, all cells divide and the mutations at that stage will be carried to the descending cells; and after the body mature, most cells stop dividing and keep the mutations from their ancestors. But new mutations will continue to appear in the after dividing cells, which could be the cause of diseases. Every neuron in the brain is different, single mutation in one neuron may becomes the seed for Alzheimer disease,  Parkinson disease, Huntington disease, and CJD......For this reason, Lodato et al. wanted to investigate the mutation pattern in single somatic neurons.

They did whole genome sequencing of 36 neurons from 3 normal adult brains, and analyzed the data on single nucleotide variants. By comparing with reference data, they found ~1500 mutations for each neuron, which are more associated with transcription regulating neuronal functions than DNA replication in cell division as in cancer cells. By comparing the mutations in each neuron, they found the neurons could be arranged into four clades clusters, each originating from the same ancestors during fetal development. The result is a beautiful tree of neurons, though the neurons are taken from a brain tissue within millimeters.

This study makes use of the new techniques to prove a concept that somatic mutations can be used to construct the linage tree of neurons, which is important to understand neurodevelopment. It also indicates somatic mutations may have more weight than germ line mutations for the neurological disorders. This method could be extended to patients' brain to search for the most possible culprit of neurological diseases. However, non-localized somatic mutation, like cancer mutation, is hard to be targeted by gene therapy.

A new therapy for memory loss?

Deep brain stimulation has been used in the treatment of several neurological diseases, such as essential tremor, Parkinson disease, dystonia, and oppressive compulsive disorder. But the exact mechanisms are unknown. A recent paper in Nature add a new disease to the catalogue.

Hao et al. reported that forniceal deep brain stimulation rescued hippocampal memory in Rett syndrome mice. Rett syndrome is a genetic disorder caused by MECP2 gene mutation, leading to a series of abnormalities including apraxia, seizure and intellectual disability. The authors studied the hippocampus-dependent memory in the mouse model of Rett syndrome, because memory deficits is the most reproducible measures in this model. One of the reasons that they use forniceal deep brain stimulation, though not mentioned in the beginning of the paper, might be that forniceal stimulation can enhance hippocampal memory in rodents. Thus their hypothesis is that forniceal stimulation can rescue the memory deficits in Rett syndrome mice.

The animals were divided into 4 groups: wild type sham, wild type stimulation, Rett syndrome sham, and Rett syndrome stimulation. They implanted the stimulation electrodes into the fibria-fornix, and recording electrodes into the dentate gyrus of the brain. Then mice in deep brain stimulation groups were treated with 130 Hz, 60 microsecond pulse for 1 hour a day for 14 days, and the mice in sham group were treated without pulsation. After 3 weeks, they performed the functional tests on these mice. They observed the changes of the fear memory, spatial learning and memory, long term potentiation, and neurogenesis. All these phenotypes were improved in stimulation group compared with sham group, not only for Rett syndrome mice but also for wild type mice. The results indicate that hippocampal neurogenesis are increased by foniceal deep brain stimulation, and thus the hippocampus-dependent memory is improved. More neurogenesis, better memory, like the treadmill experiments in mice once encourage people to run, is not new. And in this report, they may need to increase neurogenesis by another way to see if the memory tests in Rett syndrome brain are improved.

Although it's quite an interesting paper with some promise for neurological disorders that affect learning and memory, I am surprised that Nature accepted this paper.

Visible inequality causes less cooperation and wealth?

In a social network like Linkedin, would you more likely to connect with people with higher social status than you or those with lower ones than you? If you are told your coworkers earned much less than you, would you like to collaborate with them for a project? A recent paper published in Nature provides a laboratory model for questions like these.

Economic inequality exist in most human societies, whereas human have strong preference for equalities. Nishi et al. asked what may determine the inequality and what is the consequence of inequality on wealth. They displayed a networked public goods game—

1. 1462 subjects were placed in groups with a average size of 17.21.

2. Each subject was connected to an average of 5.33 neighbors.

3. The subjects were initially assigned wealth (units) to be rich, poor, or non-rich-non-poor.

4. The subjects played a cooperation game lasting 10 rounds.

5. In each round, the subjects chose to cooperate or defect with each neighbor—cooperate by reducing 50 units from their own wealth to increase their neighbors' by 100 units each; defect by doing nothing to cause no cost and benefit.

6. After the choosing cooperation or defect, the subjects were informed their neighbors' choices, they can then decide to keep or break the connection with their neighbors.

7. Some of the groups were informed the wealth situations (rich, poor, non-rich-non-poor) before the game started.

What they found—

1. Visible wealth, relative to invisible wealth, increases the inequality when the subjects knows they are unequal in the beginning.

2. Invisible wealth causes more average-wealth increase than visible wealth does.

3. Visible wealth, relative to invisible wealth, lowers overall cooperation with neighbors.

4. If the wealth are visible, the new rich in the initial equal condition are more likely to cooperate, whereas the current rich in the initial unequal condition are more likely to defect.

5. The rich keeps rich, and the poor keeps poor.

What does it mean? It means concealing wealth may increase the cooperation and reduce the inequality in the society. And initial equality, relative to initial inequality, causes more wealth increase. Are these true in the real world? It may support some phenomenons like the one mentioned in the beginning, but the real world is complicated, and there are many other factors that may affect the results. At least, it's more difficult to conceal your wealth, and go back to the initial equality now than the hunter-gather time.

On watching Steven Jobs

We watched the movie Steven Jobs today. It's a good movie with full emotional display and loyal sketch of Steven Jobs as a business creator and legendary hero.

Like it or not, Steve Jobs is adored by most human beings in the world, because he is the typical type of man representing the will of power. And most leaders in the world are among this group. Nietzsche said "Man is a rope stretched between the animal and the Overman—a rope over the abyss".  We have to admit that it's still the best explain of human existence so far. Man is not the goal but the bridge to the goals—the animal or Overman. We understand that neither goal is easy to reach. For average man, we either have the will of power, or will of no will, but in the end, we are driven by man with the will of power as a race on the Earth.

From evolutional view, it quite fits with the "Selfish gene" argued by Richard Dawkins. We are only the machines driven by genetic codes to transfer them to the next generation, for whatever purpose. Thus the will of power in itself is just the plan of genes that to expand into the whole space, like what it presents in the movie "21st century space odyssey".

The brain structure that makes mothers special

We know intuitively that women are more likely than men to pursue child care. This social task division is attributed to sexual dimorphism in the brain. The hormone difference between women and men not only forges different sexual organs, but also shapes different brain. Due to the difficulties to study human behaviors, our knowledge of sexual dimorphism is mainly based on animal experiments. In a recent Nature paper, the investigators discovered a new neural circuit that control the maternal care in mice.

Dopamine is a neurotransmitter playing essential roles in controlling voluntary movements in midbrain, short of which cause Parkinson disease; it also contributes to many behavioral processes, including mother-pup interaction. In an anatomical structure called anteroventral periventricular nucleus (AVPV) in the hypothalamus, a brain area critical in coordinating sexual dimorphism, we know that there are more dopaminergic neurons in females than males. Thus the investigators of the paper raised their hypothesis — the difference of dopaminergic neurons in AVPV of males and females may cause the sex differences in parental care.

First, they confirmed the double numbers difference of dopaminergic neurons in AVPV between male and female mice, also with more AVPV dopaminergic neurons in parental females than virgin females. Then they pharmacologically destroyed these neurons or genetically overexpressed dopamine in these neurons, or selectively activated these neurons, after which they recorded the parental behavior changes of these mice, including latency to retrieve pups and parental duration in both male and female mice, also aggressive behaviors in male mice. As expected, the ablation of AVPV dopaminergic neurons increased the pup-retrieval latency, and decreased the maternal duration, while overexpressing dopamine or activating these neurons did the opposite in female but not male mice. Unexpectedly, they found the ablation of AVPV dopaminergic neurons can increase male aggressiveness to pups, while overexpressing dopamine or activating these neurons can reduce the aggressiveness.

Next, to connect the dopaminergic neuron difference with more direct functional difference in parental behavior, they tested several possible hormones involved, including oestradiol, corticosterone, prolactin, and oxytocin. Oxytocin is the only hormone that was reduced after AVPV dopaminergic neuron ablation, which let the investigators to make their second hypothesis that AVPV dopaminergic neurons control oxytocin secretion in oxytocin-secreting neurons in paraventricular nucleus (PVN) or supraoptic nucleus (SON). By using chemical tracer and electrophysiological recording, they proved that AVPV dopaminergic neurons projected to PVN oxytocin-secreting neurons, which completes the circuit for maternal care.

These experiments used classical strategy to study anatomical and functional neural circuits, with modern molecular techniques. It could be furthered if the investigators can clarify the dopaminergic control of aggressiveness in male mice during parenting, maybe through another nucleus in amygdala.

ALDH1 and ALDH2, two faces of alcohol tolerance?

Alcoholic is a social problem in western countries but not in East Asian countries probably due to an enzyme difference for alcohol metabolism among the people. Aldehyde dehydrogenase (ALDH) is one of the key enzymes that degrade alcohol to acetic acid in the liver. There are two major isoenzymes of ALDH: ALDH1 and ALDH2. ALDH1 locates in cytosol and ALDH2 locates in mitochondria. Most Caucasians have both isoenzymes, but ~50% East Asians have normal ALDH1 and inactive ALDH2. Interestingly, low ALDH2 activity is associated with alcohol intolerance, whereas low ALDH1 activity is associated with alcoholic.  A recent paper in Science (Kim et al. (2015)) provides a possible explanation for this phenomena.

In the Science paper, the investigators elucidate an alternative pathway for GABA synthesis in midbrain dopaminergic neurons. The neurotransmission in midbrain dopaminergic neurons is essential to understand the mechanisms of Parkinson disease, a neurodegenerative disorder affecting many people. The investigators are interested in a puzzle that GABA and dopamine are co-released from dopaminergic neurons, but very few dopaminergic neurons express the classic GABA synthesis enzymes—glutamate decarboxylases (GAD65 and GAD67). Learning from another enzyme, ALDH, used by plant, frog, and glia cells to synthesize GABA, they had a hypothesis that ALDH is the enzyme for GABA synthesis in midbrain dopaminergic neurons.

First, they recorded the alterations in inhibitory postsynaptic currents (IPSC) in spiny projection neurons in the striatum of mice, which receive input signal (GABA) from midbrain dopaminergic neurons. By using GAD inhibitor or GAD knockout, they confirmed that the IPSC in spiny projection neurons are independent of GAD activity. In addition, they excluded the possibility that dopamine may activate GABA receptors on spiny projection neurons.

Next, based on previous knowledge that one isoenzyme of ALDH, ALDH1,  is cytosolic and highly expressed in the brain, they checked Aldh1a1 expression in midbrain and found it highly abundant in the terminals of midbrain dopaminergic neurons. Then they found the IPSC in spiny projection neurons were reduced with ALDH inhibitors or Aldh1a1 knockout, which supports the hypothesis that ALDH1 mediates the GABA synthesis in midbrain dopaminergic neurons.

At last, due to the association of Aldh1a1 with alcoholic and the involvement of dopaminergic system in alcoholic addiction, they continued to test the connection in a alcohol binge model in mice. They found binge drinking can reduce IPSC in spiny projection neurons, and Aldh1a1 knockout mice consume more alcohol than normal mice. It indicates that alcohol can inhibit GABA synthesis in midbrain dopaminergic neurons, and in people with ALDH1A1 variants, the GABA inhibition is abolished, which causes alcoholic behavior. 

Although both ALDH1 and ALDH2 are involved in alcoholic metabolism, ALDH1 is responsible for GABA synthesis in dopaminergic neurons, whereas ALDH2 is responsible for alcohol clearance. without ALDH1, you are more likely to be alcoholic; without ALDH2, you are more likely to be intolerable of alcohol. That said, ALDH2 is also highly expressed in the brain. Will ALDH2 variants affect the GABA synthesis and alcohol addiction? This is the limit of this paper.

“Neanderthal Man: In search of the lost genome" by Svante Pabbo

When I was a child, I was enchanted by the mysterious atmosphere of graveyards, hoping to see some skeletons, and know their lost stories. It seems I am not alone. Svante Pabbo, the Swedish molecular anthropologist is one of the most famous people to build a career on his childhood’s dream.

In a new memoir “Neanderthal Man: In search of the lostgenome", Svante Pabbo tells us a vivid story of how a curious and sentimental boy’s dream turns true. When he was a child, Svante Pabbo was attracted to Egyptian mummies and fascinated by their mysteries, hoping to solve the puzzles around the mummies one day. It was not until he was a graduate student that Svante Pabbo got the spark to analyze the genome of Egyptian mummies, when molecular biology started to boom in 1980s. Followed by a series of questions in 25 years, this initial maneuver led to his final success on sequencing the genome of Neanderthal man.

In the first chapter, Svante Pabbo described an eureka in 1996, when his student Matthias Krings decoded a fragment of the mitochondria DNA sequence from the DNA sample extracted from a 40000 years old Neanderthal Man that was excavated from the Neander Valley in Germany. He was so excited and cautious too that he did not open the champagne until he was confident with the results after several repeats. This breakthrough gave him confidence on decoding genes in ancient humans.

In the following chapters, Svante Pabbo tells his story in a chronological order, with logical questions step by step leading to the final answer. He first asked whether DNA could survive after tissue death. Answering this question, he heated the calf liver in the lab oven at 50 C for days, and got a few hundred nucleotides. Next, he asked how long the DNA could survive after tissue death. He obtained the samples of Egyptian mummies from East Germany through an anthropologist friend, and used nuclear dye to stain the cartilage and skin cells of the samples. After he found positive nuclear staining in these samples, he was sure the DNA is still there, he then extracted the DNA from these samples, and cloned the extraction into bacteria for sequencing. This experiment proved the preservation of DNA to as old as about 4000 years.

Contamination of modern DNA is the main concern of the whole business. Therefore, how to get rid of the contamination during the DNA extraction is the next question. Svante Pabbo and his students tried many ways to reduce the contamination to almost none, such as making a clean room with everything inside clean of DNA. They also developed some internal controls to solve this problem. Now the ancient DNA is cleared of contamination, they can continue to ask the next question: could 40000 years old Neanderthal man DNA be sequenced?

They first tried with a frozen Siberian mammoth about 50000 years old. If this works, the Neanderthal man will also work. They succeeded to get the mitochondrial DNA of this mammoth in 1994. Then they went on to extract DNA from Neanderthals. Now back to the scene in chapter one, they got lucky again and did get mitochondrial DNA of Neanderthals. Mitochondrial DNA has only a few hundreds nucleotides, whereas the nuclear genome has 6.4 billion nucleotides; thus, to see the big map of Neanderthal genome, they need to sequence the nuclear genome.

Most ancient DNA is degraded; therefore, it is not easy to get enough DNA to sequence nuclear DNA. Svante Pabbo examined Neandersal samples from several digging sites where the Neandersals were excavated. He could get very small amount of nuclear DNA from a few well-preserved Neandersal samples. Luckily, the DNA sequencing technology now makes it possible to use little DNA to sequence the whole genome. In 2010, Svante Pabbo and his group finally sequence the first Neandersal genome.

At last, the question comes to the human origin. Did Neandersals contribute DNA to modern human? Did modern human inbreed each other, and have gene flow to each other? By comparing the genomes of Neandersals with those of modern human of European, East Asian, African, American, and South Asian, Svente Pabbo found Neandersals shared more genetic variants with present-day humans in Europe than with present-day humans in Africa, and drew the conclusion that non-African modern human have 1-4% of their DNA originated from Neandersals 30000 years ago, when they met and inbred with each other in Middle East. After that short convention, Neandersals disappeared and modern human continued to migrate to the other part of the world and replaced the aboriginal ancient human.

Svante Pabbo’s writing is concise and logical; there is no jargon for laymen, and it is very easy to read.

Stir-fried chicken with pepper and tree fungus

Introduction:
It is an mix of meat and vegetables, and the flavor can diffuse to each other. The fungus becomes tasty with the flavor of chicken, and the chicken becomes fresher with the fragrance of pepper and fungus. The tree fungus is special for its chewer.

Ingredients:
500g chicken thighs
100g bell pepper
50g dry tree fungus
30 ml canola oil
10g bunching onion
10g ginger
10g garlic
5g sugar
5g salt
1g black pepper
1g potato starch

Preparation:
1. Cut chicken thighs into 4 cm x 4 cm pieces, put into cold water in pot, heat to boiling to remove the blood, drain the water and keep aside
2. Cut bell peppers into  4 cm x 4 cm pieces, and hydrate the tree fungus with hot water for 5 minutes
3. Chop the bunching onion, ginger, and garlic into small pieces
4. Heat the canola oil in a fry pan on the stove over high heat for 1 minute
5. Add the ginger, garlic, and bunching onion into the hot oil and stir for 1 minute
6. Add chicken thighs pieces into the pan and stir for 1 minute, then add pepper and tree fungus into the pan and stir for 1 minute
7. Add sugar, salt, and black pepper into the pan, and continue to stir for 5 minutes
8. Thicken with potato starch to finish

Stir-fried chicken heart with pepper

Introduction:
It is an easy-to-prepare dish based on the taste of chicken heart. You can add your favorite spices to adjust the final flavor. The fine texture of the heart makes it just right for chew, and the smooth muscle makes it tasty.

Ingredients:
500g chicken heart
100g bell pepper
30 ml canola oil
10g bunching onion
10g ginger
10g garlic
5g sugar
5g salt
1g black pepper
1g potato starch

Preparation:
1. Cut chicken heart into 0.5 cm thick slices, put into cold water in pot, heat to boiling to remove the blood, drain the water and keep aside
2. Cut bell pepper into 2 cm x 2 cm blocks
3. Chop the bunching onion, ginger, and garlic into small pieces
4. Heat the canola oil in a fry pan on the stove over high heat for 1 minute
5. Add the ginger, garlic, and bunching onion into the hot oil and stir for 1 minute
6. Add chicken heart pieces into the pan and stir for 1 minute, then add pepper blocks into the pan and stir for 1 minute
7. Add sugar, salt, and black pepper into the pan, and continue to stir for 5 minutes
8. Thicken with potato starch to finish

Three-cup chicken

Introduction:

This dish was said to originate from Jianxi in Song dynasty, and be modified in Taiwan to become very popular now in US. The original 3 cups include rice wine, pig fat, and soy sauce; the Taiwanese version includes rice wine, sesame oil, and soy sauce. This recipe is an integration of several popular recipes for three-cup chicken, with a special ratio of rice wine, soy sauce, and sesame oil as 4:2:1.

Ingredients:

500g chicken thighs

20ml sesame oil

10ml olive oil

45ml light soy sauce

15ml concentrated soy sauce

120ml rice wine (Shaoxing 17%)

20g bunching onion

20g Thai basil

20g ginger

20g garlic

5g sugar

1g salt

1g dry hot pepper

Preparation:

1. Cut chicken thighs into 4 cm x 4 cm pieces, put into cold water in pot, heat to boiling to remove the blood, drain the water and keep aside

2.Cut the bunching onion into 5cm long fragments, and ginger and garlic into 0.3 cm thick slices 

3. Heat the mixture of sesame oil and olive oil in a fry pan on the stove over high heat for 1 minute

4. Add the dry hot pepper and ginger into the hot oil and stir for 2 minutes or until they dark and dry, then add garlic and bunching onion, continue stirring for 1 minute

5. Add chicken thighs pieces into the pan and stir for 1 minute, then add the mixture of sugar, light and concentrated soy sauce, and rice wine

6. Heat over high heat until boiling and continue to heat with stir over mild heat for 20 or until the liquid dries

7. Add the thai basil 1 minute before the finish, with the lid covered

Stir-fried shrimp with loofah, tomato, and egg

Introduction:

It is a mixture of four different tastes: flavors of shrimp and egg, sourness of tomato, and fragrance of loofah.

Ingredients:

100g shrimp

200g loofah

100g tomato

2 eggs

60 ml canola oil

10g ginger

10g garlic

5g sugar

5g salt

1g black pepper

1g potato starch

Preparation:

1. Cut tomatoes into 1/8 slices

2. Remove the skin and seeds of loofahs and cut into 2 cm x 2 cm blocks

3. Chop the bunching onion, ginger, and garlic into small pieces

4. Heat 20ml canola oil in a fry pan on the stove over high heat for 1 minute, add two light-beating eggs into the pan, stir and take them out once they are solidified 

5. Heat 20ml canola oil in a fry pan on the stove over high heat for 1 minute, add the shrimps, flap and take them out once they turn pink 

6. Add the ginger, garlic, and bunching onion into the hot oil and stir for 1 minute

7. Add tomato and loofah blocks into the pan and stir for 1 minute

8. Add sugar, salt, and black pepper into the pan, and continue to stir for 3 minutes

9. Add the prepared eggs and shrimps into the pan and stir with the loofah and tomato for 3 minutes
10. Thicken with potato starch to finish

Ratatouille

Stir-fried chicken gizzards with squash

Introduction:
Chicken gizzard is very popular food around the world except in Western countries. Its elastic texture makes bitting fun. The squash adds extra flavors of vegetables. You can add your favorite spices to adjust the taste.

Ingredients:
500g chicken gizzards
250g squash
30 ml canola oil
10g bunching onion
10g ginger
10g garlic
5g sugar
5g salt
1g dry hot pepper
10ml rice wine (Shaoxing 17%)
1g black pepper
1g potato starch

Preparation:
1. Cut chicken gizzards into 0.3 cm thick slices, put into cold water in pot, heat to boiling to remove the blood, drain the water and keep aside
2. Cut squash into 0.3 cm thick round slices
3. Chop the bunching onion, ginger, and garlic into small pieces
4. Heat the canola oil in a fry pan on the stove over high heat for 1 minute
5. Add dry hot pepper powder into the hot oil and stir for 2 minutes, then add the ginger, garlic, and bunching onion into the hot oil and stir for 1 minute
6. Add chicken gizzards pieces into the pan and stir for 1 minute, then add rice wine, and stir for 5 minutes
7. Add squash slices into the pan and stir for 1 minute
7. Add sugar, salt, and black pepper into the pan, and continue to stir for 3 minutes
8. Thicken with potato starch to finish

Kung-Pao Cauliflower

Introduction:
Cauliflower can be cooked alone with its own fragrance. Peanuts and pork meat add extra flavors. Peanuts are usually cooked with chicken (Kung-Pao chicken), which is said to be invented by a governor of Qing dynasty named Baozhen Ding, and Kung-Pao is his official title. The peanuts in this dish makes it special, so I borrow it to use it in the cauliflower.

Ingredients:
500g Cauliflower
10g fried peanuts
10g grinding pork meat
30 ml canola oil
10g bunching onion
10g ginger
10g garlic
5g sugar
5g salt
1g dry hot pepper
1g black pepper
1g potato starch

Preparation:
1. Split cauliflower into 2 cm x 2 cm pieces by hands, and add into boiling water for 3 minutes before taking out and draining the water
2. Prepare fried peanuts
3. Chop the bunching onion, ginger, and garlic into small pieces
4. Heat the canola oil in a fry pan on the stove over high heat for 1 minute
5. Add dry hot pepper powder into the hot oil and stir for 2 minutes, then add the ginger, garlic, and bunching onion into the hot oil and stir for 1 minute
6. Add grinding pork meat into the pan and stir for 2 minutes
7. Add prepared cauliflower into the pan and stir for 1 minute
7. Add sugar, salt, and black pepper into the pan, and continue to stir for 5 minutes
8. Thicken with potato starch to finish

Omelette

Introduction:

It is an easy dish for breakfast, but may vary a lot from different preparations. 

Ingredients:

2 eggs

10g tomato

10g spinach

5g peas

10g mushrooms

10g onion

10g bell pepper

0.5g dry basil

0.5g dry thyme

1g parmesan cheese

1g Sriracha hot chili sauce

2g salt

1g black pepper

10ml whole milk

1 leave of fresh basil

5g lobster meat from steamed lobster

30 ml olive oil

Preparation:

1. Chop onion, bell pepper, mushroom, spinach, tomato, into small pieces

2. Crack 2 eggs into a mixing bowel, and beat well with chopsticks

3. Add the chopped vegetables, peas, parmesan cheese, dry thyme, dry basil, salt, black pepper, hot chili sauce, and milk into the eggs to stir well 

4. Heat the olive oil in a fry pan on the stove over high heat for 1 minute, then turn to mild heat

5. Spread the prepared mixture into the hot oil evenly, wait for 10 seconds for the mixture to firm a little and use spatula to direct the left wet mixture to the sides

6. Wait for 1 minute or until the mixture is 80% firm

7. Fold the mixture into a plate, decorate with the lobster meat slices, basil leave, and hot chili sauce